The importance of maturation factors in 30S ribosomal subunit assembly
نویسنده
چکیده
.......................................................................................... 5 SAMMANFATTNING PÅ SVENSKA ............................................... 7 PAPERS IN THIS THESIS .................................................................. 9 THE RIBOSOME ............................................................................... 10 The coordinated assembly of a ribosome ...................................... 11 The process of translation .............................................................. 11 RIBOSOMAL RNA (rRNA) .............................................................. 12 Regulation of rRNA transcription ................................................. 12 Antitermination ............................................................................. 13 Stringent response ......................................................................... 13 Growth rate dependent regulation ................................................. 14 Maturation of rRNA ....................................................................... 15 Processing of rRNA ...................................................................... 15 Modification of rRNA ................................................................... 15 Folding of 16S rRNA .................................................................... 16 THE r-PROTEINS ...................................................................... 17 Modification of r-proteins .............................................................. 17 Regulation of r-protein synthesis ................................................... 18 RIBOSOME ASSEMBLY FACTORS .............................................. 18 RNA chaperones, RNA helicases and ribosome dependent GTPases............................................................................................. 19 Ribosome assembly GTPases ......................................................... 20 The ribosome maturation factors. ................................................. 21 IN VITRO ASSEMBLY OF THE 30S RIBOSOMAL SUBUNIT .. 24
منابع مشابه
RsgA couples the maturation state of the 30S ribosomal decoding center to activation of its GTPase pocket
During 30S ribosomal subunit biogenesis, assembly factors are believed to prevent accumulation of misfolded intermediate states of low free energy that slowly convert into mature 30S subunits, namely, kinetically trapped particles. Among the assembly factors, the circularly permuted GTPase, RsgA, plays a crucial role in the maturation of the 30S decoding center. Here, directed hydroxyl radical ...
متن کاملStructural basis for the function of a small GTPase RsgA on the 30S ribosomal subunit maturation revealed by cryoelectron microscopy.
The bacterial RsgA, a circularly permutated GTPase, whose GTPase activity is dependent on the 30S ribosomal subunit, is a late-stage ribosome biogenesis factor involved in the 30S subunit maturation. The role of RsgA is to release another 30S biogenesis factor, RbfA, from the mature 30S subunit in a GTP-dependent manner. Using cryoelectron microscopy, we have determined the structure of the 30S...
متن کاملInteraction of Era with the 30S ribosomal subunit implications for 30S subunit assembly.
Era (E. coliRas-like protein) is a highly conserved and essential GTPase in bacteria. It binds to the 16S ribosomal RNA (rRNA) of the small (30S) ribosomal subunit, and its depletion leads to accumulation of an unprocessed precursor of the 16S rRNA. We have obtained a three-dimensional cryo-electron microscopic map of the Thermus thermophilus 30S-Era complex. Era binds in the cleft between the ...
متن کاملDissecting the in vivo assembly of the 30S ribosomal subunit reveals the role of RimM and general features of the assembly process
Ribosome biogenesis is a tightly regulated, multi-stepped process. The assembly of ribosomal subunits is a central step of the complex biogenesis process, involving nearly 30 protein factors in vivo in bacteria. Although the assembly process has been extensively studied in vitro for over 40 years, very limited information is known for the in vivo process and specific roles of assembly factors. ...
متن کاملAssembly of the 30S ribosomal subunit: positioning ribosomal protein S13 in the S7 assembly branch.
Studies of Escherichia coli 30S ribosomal subunit assembly have revealed a hierarchical and cooperative association of ribosomal proteins with 16S ribosomal RNA; these results have been used to compile an in vitro 30S subunit assembly map. In single protein addition and omission studies, ribosomal protein S13 was shown to be dependent on the prior association of ribosomal protein S20 for bindin...
متن کامل